Berkhamsted were dealt a big dent in their quest for the SSML Premier Division title when they were beaten 1-0 by promotion rivals Hanwell Town at Broadwater.
The Comrades remain top of the division, but Hanwell are now only four points behind Mick Vipond’s side with six games in hand.
The game was settled from the penalty spot in the first half when Berko defender Ed Canham rushed towards the ball in the area and appeared to put his hand up to protect his face.
He was struck on the hand with the ball and the match referee ruled it was handball rather than a legitimate attempt to protect himself.
Hanwell loanee Dominic Rhone stepped up to take the resulting spot-kick and coolly sent Comrades keeper Carl Tasker the wrong way to net what proved to be the winning goal.
There were plenty chances in a physical match with Connor Calcutt, Jamie Gavin and Frankie Jowle all going close for Berko.
At the Canal End, Charlie Fanner produced a fine save to keep out a looping ball when it looked like he was beaten, while Berko had a similar shout for a penalty but saw their appeals waved away.
Hanwell substitute Eion Casey had the chance to seal the win with his first touch but Tasker saved his shot as well as the follow up effort.
Mick Vipond sent on three forward substitutes to try and rescue the game but it was to no avail.